RNA
gard-337
Part:BBa_K3714000
Designed by: Yuxin Lu Group: iGEM21_SJTU-BioX-Shanghai (2021-08-30)
Gardiquimod biosensor 337
The template of a newly found self-cleaving aptazyme[1], which can keep itself intact with gardiquimod present, and catalyze the self-cleavage reaction in the absence of gardiquimod. This part could be used as a switch for gene expression controlled by gardiquimod. The feature "Aptazyme" is necessary, and two A-rich oligonucleotides are added at both terminal to avoid unwanted secondary structure.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1] Townshend, B., Xiang, J. S., Manzanarez, G., Hayden, E. J. & Smolke, C. D. A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors. Nature Communications 12, 1437, doi:10.1038/s41467-021-21716-0 (2021).
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Categories
Parameters
//rna/aptazyme
efficiency | 10 μM gardiquimod inhibits 94.4% cleavage[1] |