Plasmid_Backbone

Part:BBa_K3945014

Designed by: Dr. Schryverss Lab   Group: iGEM21_Calgary   (2021-10-20)
Revision as of 05:32, 21 October 2021 by Dewuni D (Talk | contribs)

Xpress: T7-Compatible Expression Vector for E. coli with A GST solubility Tag


Usage and Biology

Design

Experimental Workflow

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 1908
    Illegal PstI site found at 2955
    Illegal PstI site found at 3842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal PstI site found at 2955
    Illegal PstI site found at 3842
    Illegal NotI site found at 3
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal BamHI site found at 757
    Illegal XhoI site found at 912
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 1908
    Illegal PstI site found at 2955
    Illegal PstI site found at 3842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 1908
    Illegal PstI site found at 2955
    Illegal PstI site found at 3842
    Illegal NgoMIV site found at 2201
    Illegal NgoMIV site found at 2598
    Illegal NgoMIV site found at 2608
    Illegal NgoMIV site found at 2734
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3694
    Illegal SapI.rc site found at 160
    Illegal SapI.rc site found at 2705

References

[edit]
Categories
Parameters
None