Composite

Part:BBa_K3893008

Designed by: Tannya Sandoval   Group: iGEM21_Ecuador   (2021-09-12)
Revision as of 00:47, 22 October 2021 by Acarmas6 (Talk | contribs)


Up part of cassette for dsRNA constitutive production 1

Part of Modular Platform for dsRNA production

Upper part of the cassette for constitutive dsRNA production, which is composed of a terminator (BBa_K3893007), promoter T7 (BBa_I712074), RBS (BBa_B0034) andGFP transcription unit that functions as a dropout (BBa_K3893017).

Assembly system


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 165
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 921
    Illegal SpeI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 165
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 837


[edit]
Categories
Parameters
None