Coding

Part:BBa_K3757005

Designed by: Erik Zimmer   Group: iGEM21_Tuebingen   (2021-09-26)
Revision as of 14:27, 26 September 2021 by Registry (Talk | contribs)

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c_KR-12_1

Cyclotide MCoTI-II with Trypsin inhibitor activity from Momordica cochinchinensis. The antimicrobial fragment KR-12 of the human cathelicidin LL-37 is grafted into loop 1 of the cyclotide, a His6-tag is grafted into loop 5. This part can be cloned into Oak1 precursor and co-expressed with CtAEP1 to yield as a cyclic peptide in Nicotiana benthamiana.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None