Coding

Part:BBa_K175000

Designed by: Calin Plesa   Group: iGEM09_TUDelft   (2009-07-05)
Revision as of 18:37, 5 July 2009 by Cplesa (Talk | contribs)

trbC (pilin) of IncP beta R751 plasmid

The DNA relaxase TraI of the IncP beta plasmid R751. Essential part of conjugative transfer. This part nicks oriT. It should be placed on a message plasmid along with oriT, and will enable transfer of the message plasmid in the presence of a modified R751 helper plasmid such as BBa_I714037.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//dna/conjugation
//function/conjugation
Parameters
directionForward
n/atrbC (pilin) of IncP beta R751 plasmid