Composite

Part:BBa_K3739034

Designed by: Ziyan Han   Group: iGEM21_XMU-China   (2021-09-09)
Revision as of 17:47, 9 September 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


J23100-B0030-Aly01-his-3Spycatcher-B0010

"SpyTag and SpyCatcher are able to form isopeptide bond with the other without any assistant, Aly01 is a signal peptide for secretion, LecA is a sugar binding protein."


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1490
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 203


[edit]
Categories
Parameters
None