Coding

Part:BBa_K4046000

Designed by: Jessica Shah   Group: iGEM21_Duke   (2021-10-18)
Revision as of 20:11, 21 October 2021 by Shahjessica (Talk | contribs)


FLAG-NLS-DhdR

This is a modified version of the transcriptional repression factor DhdR, which is responsive to the oncometabolite D-2-HG. DhdR is a transcriptional repression factor isolated from the bacteria Achromobacter denitrificans . The sequence was human codon optimized for expression in mammalian cells. Additionally, the sequence had a nuclear localization sequence attached to the end, for transport into the nucleus as part of our genetic reporter system, and a FLAG tag attached to the end.

A schematic for the operation of our system is shown below. T--Duke--DhdrSchematic.png

Figure 1: This figure outlines the mechanism of the interaction between the DhdR allosteric transcription factor and the dhdO binding site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 310
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 186
    Illegal BsaI site found at 303


[edit]
Categories
Parameters
None