Part:BBa_K3790231
T7 gene product 2 controlled by pBAD/araC
Introduction
In order to turn the host bacteria into ‘factories’ that concentrate on manufacturing parts of the virus, some phages have the ability to shut off the endogenous transcription of host bacteria. As for T7 phage, there are three gene products collaboratively realizing the function, which are gene product (gp) 0.7, gp2 and gp5.7[1].
Gp2 is an early phage gene, which means they are transcribed by the host's RNA polymerase at the very beginning of the infection process[2].
Usage and Biology
Gp2 is a small protein that can bind to both the 1.1 domain of σ70 factor and the β’ subunit of the host's RNAP[3], results in effective inhibiting of the transcription initiated by σ70-RNAP complex.
This part can be used to generate gp2 under arabinose induction.
Experimental Results
Gp2 was eliminated by us because gp5.7 is a better choice. For more details, please visit: https://parts.igem.org/wiki/index.php?title=Part:BBa_K3790050
Reference
- ↑ T7 phage factor required for managing RpoS in Escherichia coli. Tabib-Salazar A, Liu B, Barker D, Burchell L, Qimron U, Matthews SJ, Wigneshweraraj S. Proc Natl Acad Sci U S A, 2018 Jun 5;115(23):E5353-E5362. PMID:29789383
- ↑ Roles of the early genes of bacteriophage T7 in shutoff of host macromolecular synthesis. McAllister WT, Barrett CL. J Virol, 1977 Sep;23(3):543-53. PMID:330878
- ↑ Structural and mechanistic basis for the inhibition of Escherichia coli RNA polymerase by T7 Gp2. James E, Liu M, Sheppard C, Mekler V, Cámara B, Liu B, Simpson P, Cota E, Severinov K, Matthews S, Wigneshweraraj S. Mol Cell, 2012 Sep 14;47(5):755-66. PMID:22819324
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1335
Illegal SapI site found at 961
None |