Temporary

Part:BBa_K3726080

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)
Revision as of 19:22, 8 October 2021 by Sbarraga (Talk | contribs)


P_PrbcL-m36

This part corresponds to a strong constitutive promoter for cyanobacteria. The promoter sequence corresponds with a modified version of the native PCC11801 phycocyanin β-1 subunit promoter.

Despite being a cyanobacterial promoter, it has shown high expression levels also in E. Coli.

The promoter was firstly documented by P. Wangikar et al. ("A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.), obtained via the screening of a library of promoter variants created by error-prone PCR.

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 225
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None