DNA
Part:BBa_K3791012:Design
Designed by: Laura Sánchez Ruiz, Auba Fuster Palŕ Group: iGEM21_UPF_Barcelona (2021-10-10)
Revision as of 22:33, 21 October 2021 by Laurasanchezruiz (Talk | contribs)
gRNA Erythromycin construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The repeat sequence must be placed before the spacer sequence, that is, in the 5â extreme. It is also required to have a length between 18 and 24 nucleotides, in this case adjusting it to a consensus G-C content of approximately 50%.
Source
The repeat and the T7 promoter sequences were extracted from the parts registry (BBa_K2927006) (BBa_K1614000), whereas the spacer one was designed with the constraint that it had to be complementary to the sequence of the erythromycin-resistance gene aiming to be detected (as explained in its own part page: BBa_K3791002).