![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3739067
Designed by: Shichen Geng Group: iGEM21_XMU-China (2021-09-09)
J23100-B0034-phnC-B0034-phnD-J23100-B0034-phnE1-B0034-phnE2
Phn system is a gene cluster for organophosphorus transportation and degradation in many microorganisms. Sinorhizobium meliloti 1021 encodes ABC transporter by phnEE, phnC,phnD gene. This transporter can transport glyphosate to cytoplasm. The phnC gene encodes ATP-binding protein and the phnD encodes phosphonate binding protein of the ABC transporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1848
Illegal NheI site found at 1871
Illegal NheI site found at 2898 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1202
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 173
Illegal NgoMIV site found at 242
Illegal NgoMIV site found at 481
Illegal NgoMIV site found at 485
Illegal NgoMIV site found at 866
Illegal NgoMIV site found at 890
Illegal NgoMIV site found at 1501
Illegal AgeI site found at 1759
Illegal AgeI site found at 4050 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 253
Illegal BsaI site found at 1104
Illegal BsaI.rc site found at 1438
Illegal SapI site found at 940
Illegal SapI.rc site found at 419
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Categories
Parameters
None |