Part:BBa_K3736001

constitutive promoter+ FourU + luxR + Terminator*2

constitutive promoter+ FourU + luxR + Terminator*2

Gene Construct of DenTeeth

  We incorporate the whole part into E. coli BL21(DE3). We did colony PCR and digest to check its genotype.

Figure 1. Quorum sensing sequence + Restoration sequence + Sterilization sequence. M :1kb DNA ladder, 1 : Constitutive promoter + RBS + LuxR + Term. +Term. + Ptet + RBS + BMP2 + RBS + STATH + RBS + GFP + Term. + Term. + Plux + RBS + LL-37 + RBS + mRFP + RBS + tetR + Term. + Term. (4685 b.p.)</i>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 890
  Illegal NgoMIV site found at 1011
  
• 1000
  COMPATIBLE WITH RFC[1000]