DNA

Part:BBa_K3905018:Design

Designed by: Peter Heywood   Group: iGEM21_City_of_London_UK   (2021-10-07)
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517-5 T7p* Probe 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ensured the structure formed when both probes have annealed to the miRNA have a high probability to have unbound ends. We designed a python script to do this using Nupack's API. We ensured GC content is 40-60%



Source

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References