Coding

Part:BBa_K4006004:Design

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-20)
Revision as of 18:05, 20 October 2021 by Registry (Talk | contribs)

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MT-6XHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5
    Illegal SapI.rc site found at 233


Design Notes

The 6X-His tag was added to the N terminal of the protein coding sequence to ensure there was no interaction with the functional domain of the folded protein.


Source

Part BBa_K3275000 - Human MT sequence

References