Coding

Part:BBa_K3739101

Designed by: Xiaoyu Wang   Group: iGEM21_XMU-China   (2021-09-29)
Revision as of 17:43, 29 September 2021 by Registry (Talk | contribs)

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his-SpyTag-GFP

SpyTag protein is fused with GFP to verify INPNC's function that it can anchor proteins onto membranes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None