Coding
lacZa-ccdB

Part:BBa_K176003

Designed by: Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-07-03)
Revision as of 16:39, 3 July 2009 by Horigen (Talk | contribs)

lacZalpha-ccdB coding sequence

A fusion protein of lacZalpha(I732006) and ccdB(K145151). It should have the function of ccdB to cause E. coli cells to die, but have less toxicity because of the lacZalpha part. It should also have the function of lacZalpha as a reporter for measurement.

This part is similar to J32026 but the multiple cloning sites are eliminated. It is constructed by PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You). See "Part Design" for more details.

K176003 MutiAlin.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387


[edit]
Categories
Parameters
n/alacZalpha-ccdB coding sequence