Part:BBa_K3720003

Firefly luciferase with pCooM, RBS (weak), and double terminator

pCooM is CooA-dependent promoter originates from Rhodospirillum rubrum. Since CooA binds to carbon monoxide and activates pCooM, it is possible to create a light emitting mechanism that reacts with carbon monoxide when this part used in combination with CooA.

In fact, we introduced a plasmid incorporating this part into our E. coli K-12 strain. We then mixed 40㎕ of liquid culture medium of this E. coli with 1.0㎕ of D-luciferin (15 mg/ml) and subsequently injected CO and measured the luminescence. Although quantitative measurements could not be conducted because there is no proper device in our lab for observing bioluminescence. However, as shown in the picture, we can see with the naked eye that the color of the tube injected with carbon monoxide has changed.

Fig.1 Color changes in bacterial culture
Left: LB medium+luciferin Right: LB medium+luciferin+CO gas

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 900