Coding
Part:BBa_K3739027
Designed by: Ziyan Han Group: iGEM21_XMU-China (2021-09-08)
LamB1-LC1KR2-LamB2-his
We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use BBa_K3739057 to construct the expression system and anchor LCIKR-2 on the surface of VnDX.
Biology
LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.
Usage
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 612
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 760
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Categories
Parameters
None |