Coding
COMT

Part:BBa_I742107

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-19)
Revision as of 14:27, 9 October 2021 by Nikitaigem bhopal (Talk | contribs)

COMT

COMT coding sequence

Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring, converting caffeic acid to ferulic acid) from Medicago sativa (alfalfa). This gene was synthesised by Geneart.


IISER_BHOPAL 2021

Usage and Biology

It has been found that strong downregulation of COMT in transgenic alfalfa plants decrease the lignin amount and a reduction in the number of units of guaiacyl (G) lignin with a total loss of syringyl (S) units in monomeric and dimeric lignin degradation products.[1]

References

1.Guo, D., Chen, F., Inoue, K., Blount, J. W., & Dixon, R. A. (2001). Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin. The Plant Cell, 13(1), 73. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 577


[edit]
Categories
//function/biosynthesis/odorant
//function/odor
Parameters
None