Composite

Part:BBa_K3739087

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-10-20)
Revision as of 16:48, 20 October 2021 by Registry (Talk | contribs)

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K525998(T7-RBS)-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 200
    Illegal NgoMIV site found at 636
    Illegal NgoMIV site found at 1371
    Illegal NgoMIV site found at 1607
    Illegal AgeI site found at 273
    Illegal AgeI site found at 1100
    Illegal AgeI site found at 1596
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None