Part:BBa_K3905008
T7 / gen2 517-5p Toehold Switch / Luciferase / Double Terminator
This composite part is used for testing the presence of hsa Micro-RNA 517-5p. It has a T7 promoter for expression of functional RNA (Part:BBa_K1614000). This is followed by the code for our toehold switch (Part:BBa_K3905004). This Toehold Switch is activated by hsa-MiRNA 517-5p. The micro RNA 517-5p alone acts as the trigger for the toehold switch and causes the riboregulator to unfold, exposing the RBS and start codon. Placing a reporter protein downstream of this part will mean that when miRNA 517-5p is present in high enough quantities, the protein will be translated and the reporter protein can be observed. Our chosen reporter protein is luciferase (Part:BBa_I712019). When Luciferin is added, luciferase will catalyse a reaction that produces light. This light can be detected with a luminometer to affirm a positive test.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 41
Illegal XbaI site found at 79 - 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 41
Illegal XbaI site found at 79 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 41
Illegal XbaI site found at 79 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 922
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