Composite

Part:BBa_K3794001

Designed by: Harshraj Bumia   Group: iGEM21_KCL_UK   (2021-09-30)
Revision as of 18:16, 21 October 2021 by Harshbumia (Talk | contribs)


PVFP-5 Composite

This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein, BBa_K3794000, in E.coli. It is under the control of the lac promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021.

Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column.

This composite part / translational unit does not contain a terminator, therefore a plasmid, such as pSB1A3, containing a terminator region or an addition terminator part from the iGEM registry is required.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None