Composite

Part:BBa_K3777026

Designed by: SiYuan Peng   Group: iGEM21_OUC-China   (2021-10-01)
Revision as of 18:49, 21 October 2021 by Wuti (Talk | contribs)


KB2-mphR-ermC-T7(mphO)-3WJdB-PmphR-sgRNA

Basic biosensor device for erythromycin detection.

Usage and Biology
Compared to our another mphR-ermC-T7(mphO)-3WJdB(BBa_K3777021),it adds a binding site which can be regonized by dcas9 proteins and sgRNA.(Fig 1)

When Ery was absent, mphR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene. But BS2 will be regonized and cut by dca9 proteins and sgRNA leading to lower fluorescence expression. 800px-KB2-mphR-ermC-T7%28mphO%29-3WJdB-PmphR-sgRNA.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 260
    Illegal NheI site found at 283
    Illegal NheI site found at 1599
    Illegal NheI site found at 2015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1493
    Illegal XhoI site found at 121
    Illegal XhoI site found at 1144
    Illegal XhoI site found at 2139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None