Coding

Part:BBa_K3794000

Designed by: Harshraj Bumia   Group: iGEM21_KCL_UK   (2021-09-30)
Revision as of 18:09, 21 October 2021 by Harshbumia (Talk | contribs)


PVFP-5

Perna viridis foot protein-5 (PVFP-5), is one of the first adhesive proteins secreted by the mussel foot from the Perna viridis mussel species. PVFP-5 is an adhesive protein that can be utilised in biomedical and therapeutic applications due to its low immunogenicity and non-cytotoxicity in the body. It can be used as a bioadhesive coating for medical implants, such as a 3D printed scaffold / nerve channel for axonal regeneration. There is an encoded N-terminal 6xHis tag and TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation. This part has been synthesised by IDT as a composite, which can be found at (with an upstream LacI Regulator (BBa_R0010)).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None