Composite

Part:BBa_K3739036

Designed by: Ziyan Han   Group: iGEM21_XMU-China   (2021-09-09)
Revision as of 20:22, 9 September 2021 by Registry (Talk | contribs)

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J23100-B0030-Aly01-his-CBM-hutH-B0010

Aly01 is a signal peptide from the alginate lyase of V.natriegens, his-tag enable us to purify the linking protein, CBM can bind to cellulose and hutH is a histidine transaminase which converts histidine into urocanic acid


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 679
    Illegal NgoMIV site found at 1115
    Illegal NgoMIV site found at 1850
    Illegal NgoMIV site found at 2086
    Illegal AgeI site found at 137
    Illegal AgeI site found at 425
    Illegal AgeI site found at 515
    Illegal AgeI site found at 752
    Illegal AgeI site found at 1579
    Illegal AgeI site found at 2075
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None