Composite
Part:BBa_K3739036
Designed by: Ziyan Han Group: iGEM21_XMU-China (2021-09-09)
J23100-B0030-Aly01-his-CBM-hutH-B0010
Aly01 is a signal peptide from the alginate lyase of V.natriegens, his-tag enable us to purify the linking protein, CBM can bind to cellulose and hutH is a histidine transaminase which converts histidine into urocanic acid
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 679
Illegal NgoMIV site found at 1115
Illegal NgoMIV site found at 1850
Illegal NgoMIV site found at 2086
Illegal AgeI site found at 137
Illegal AgeI site found at 425
Illegal AgeI site found at 515
Illegal AgeI site found at 752
Illegal AgeI site found at 1579
Illegal AgeI site found at 2075 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |