Composite
Bst-DbpA
Part:BBa_K3790150
Designed by: Guanqiao Chi Group: iGEM21_Fudan (2021-10-16)
Bst-(G2S)3-DbpA
Introduction
The fusion protein obtained by linking the C-terminus of Bst Pol to the N-terminal of DbpA via a (G2S)3 linker
Usage and Biology
The fusion protein obtained by linking the C-terminus of Bst Pol to the N-terminal of DbpA via a (G2S)3 linker. Moreover, in our experimental expectation, the enzymatic activity of this fusion protein is increased compared to wild-type Bst Pol.
Experimental Results
The length of Bst-(G2S)3-DbpA DNA was 2013 bp, which is approximately 2020 bp after adding homology arms to both ends for PCR cloning. We isolated the DNA of interest by gel extraction for subsequent reactions.
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//cds
None |