Coding
Sso10b
Part:BBa_K3790003
Designed by: Guanqiao Chi Group: iGEM21_Fudan (2021-10-01)
Sso10b
Introduction
sso10b is a double-stranded binding protein from Sulfolobus solfataricus (the same species as where Sso7d from), which is close to Sso7d in length and structure. We speculate that it may have a similar function to enhance DNA polymerase activity as Sso7d.
Usage and Biology
Experimental Results
The length of albA1 DNA was 288 bp, which is approximately 300 bp after adding homology arms to both ends for PCR cloning. We isolated the DNA of interest by gel extraction for subsequent reactions.
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//cds
None |