Part:BBa_K3735888

SeNPs production parts

In order to explore the optimal conditions for the production of SeNPs for BBa_K3735004, we used the orthogonal experimental model-Response surface model(more details see CO Model). We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.

Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16°C,30°C,37°C and selenite concentration are 12mM,15mM,18mM.

Second according to preliminary results and setting gradients to explore the interaction between three different types of conditions—pH 6, 7, and 8, temperature 16。C, 30。C, and 37。C, selenite concentration 12, 15, and 18mM, and conducted experiments as shown in the Fig.2.</p>

Fig.2 Response surface experiment of SeNPs synthesis.

We used response surface model to simulate the optimal SeNPs production conditions (details see CO Model（此处添加超链接至相关页面）). Finally, we obtained the optimal conditions and maximum yield as follows： <p>Solutions

Fig.3 the final product of our project.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
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• 21
  INCOMPATIBLE WITH RFC[21]

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• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 329
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  Illegal AgeI site found at 671
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• 1000
  COMPATIBLE WITH RFC[1000]