Coding

Part:BBa_K3788025

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-30)
Revision as of 06:58, 30 September 2021 by Registry (Talk | contribs)

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pelB_sfGFP

BBa_K3788025 is coding for pelB and sfGFP. pelB is a leader sequence, it allows the protein to join the periplasm via the SEC system (unfolded proteins). In this contruction sfGFP permit to understand the mechanism, this protein can at the end be replaced by an other protein. sfGFP is supposed to be address to the preriplasm thanks to pelB leader and get his folded form into the periplasm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 79


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Categories
Parameters
None