Reporter

Part:BBa_K3814004:Design

Designed by: Mark Somerville   Group: iGEM21_Sydney_Australia   (2021-10-01)
Revision as of 13:17, 1 October 2021 by Registry (Talk | contribs)

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free-use GFP (fuGFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction enzymes were removed to minimise off-target effects. Substitute bases were chosen to most closely match the natural codon frequency in bacteria.


Source

Coleman Lab


References