Other
Part:BBa_K3814003:Design
Designed by: Simon Tang Group: iGEM21_Sydney_Australia (2021-10-01)
RiboJ ribozyme insulator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Restriction enzymes were removed to minimise off-target effects. Substitute bases were chosen to most closely match the natural codon frequency in bacteria.
Source
https://doi.org/10.1101/285866