![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4011018
Designed by: Ziteng Feng Group: iGEM21_LINKS_China (2021-10-21)
pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1
pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1 is an expression cassette in S. cerevisiae capable of expressing AeAT9 and SaACS2, which will synthesize ethyl-acetate from acetate and ethanol. SaACS2 will convert acetate into acetyl-CoA, and AeAT9 will convert acetyl-CoA and ethanol to ethyl-acetate. pTEF1-SaACS2-tADH1 BBa_K4011016 and pRPL8B-AeAT9-tSSA1 BBa_K4011017 were used to construct pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3375
Illegal BglII site found at 3582
Illegal BglII site found at 4200
Illegal XhoI site found at 2426
Illegal XhoI site found at 4699 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3609
Illegal AgeI site found at 1987 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2412
Illegal BsaI site found at 4685
Illegal BsaI.rc site found at 205
Illegal BsaI.rc site found at 2662
Illegal BsaI.rc site found at 4935
Illegal SapI.rc site found at 4447
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Categories
Parameters
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