Composite

Part:BBa_K4011018

Designed by: Ziteng Feng   Group: iGEM21_LINKS_China   (2021-10-21)
Revision as of 12:57, 21 October 2021 by Registry (Talk | contribs)

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pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1

pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1 is an expression cassette in S. cerevisiae capable of expressing AeAT9 and SaACS2, which will synthesize ethyl-acetate from acetate and ethanol. SaACS2 will convert acetate into acetyl-CoA, and AeAT9 will convert acetyl-CoA and ethanol to ethyl-acetate. pTEF1-SaACS2-tADH1 BBa_K4011016 and pRPL8B-AeAT9-tSSA1 BBa_K4011017 were used to construct pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3375
    Illegal BglII site found at 3582
    Illegal BglII site found at 4200
    Illegal XhoI site found at 2426
    Illegal XhoI site found at 4699
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3609
    Illegal AgeI site found at 1987
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2412
    Illegal BsaI site found at 4685
    Illegal BsaI.rc site found at 205
    Illegal BsaI.rc site found at 2662
    Illegal BsaI.rc site found at 4935
    Illegal SapI.rc site found at 4447


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