Composite

Part:BBa_K4011017

Designed by: Zhuhan Liu   Group: iGEM21_LINKS_China   (2021-10-21)
Revision as of 12:53, 21 October 2021 by Registry (Talk | contribs)

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pRPL8B-AeAT9-tSSA1

pRPL8B-AeAT9-tSSA1 is an expression cassette in S. cerevisiae capable of expressing AeAT9, which will synthesize ethyl-acetate from acetyl-CoA and ethanol. This is the second step into our pathway to turn acetate and ethanol into ethyl-acetate. pRPL8B BBa_K4011020, AeAT9 BBa_K4011006, and tSSA1 BBa_K4012020 were used to construct pRPL8B-AeAT9-tSSA1 using Golden Gate assembly. This part was used to construct pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1 BBa_K4011018.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 705
    Illegal BglII site found at 912
    Illegal BglII site found at 1530
    Illegal XhoI site found at 2029
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2015
    Illegal BsaI.rc site found at 2265
    Illegal SapI.rc site found at 1777


[edit]
Categories
Parameters
None