Composite

Part:BBa_K3805110

Designed by: Yue Tang   Group: iGEM21_BNU-China   (2021-10-01)
Revision as of 15:24, 1 October 2021 by Registry (Talk | contribs)

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mRNA switch

By fusing proinsulin with AraC, when the fusion protein is normally expressed, that is, when insulin is normally expressed, the fusion protein inhibits the opening of PBAD promoter and the expression of its downstream pathway. On the contrary, when the fusion protein is not expressed, this part can reflect the presence or absence of the target product.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 40
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 58
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None