Part:BBa_K4012013
CsF3H (Camellia sinensis flavanone 3-hydroxylase)
CsF3H is a coding sequence that codes for flavanone 3-hydroxylase, or called Flavonol synthase, in Camellia sinensis. It is dioxygenase involved in the flavonoid biosynthesis which has ligand with iron, metal-binding and Vitamin C. In nature, the enzyme expressed in young seedlings (at protein level). Expressed in roots, emerging leaves, shoot-root transition zone, trichomes, flowers and siliques. In cotyledons, expressed mostly on the adaxial side and only in guard cells on the abaxial side. The flavanone 3-hydroxylase catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin. In vitro catalyzes the oxidation of both enantiomers of naringenin to give both cis- and trans-dihydrokaempferol. In term of its catalytic activity, 2-oxoglutarate + a (2R,3R)-dihydroflavonol + O2 = a flavonol(naringenin) + CO2 + H2O + succinate In our project, the sequence will express flavanone 3-hydroxylase to convert naringenin into dihydroflavonol.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 160
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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