Composite

Part:BBa_K4023003

Designed by: Jiachen Lin   Group: iGEM21_Washington   (2021-10-21)
Revision as of 12:20, 21 October 2021 by Lnjc (Talk | contribs)


glpF-rbs-modified MTIA

This is a bicistronic gene containing a glpF, a ribosome binding site and a modified MTIA. This gene is designed to coexpress the glycerol uptake facilitator protein (coded by glpF) with the modified metallothionein IA protein. The glycerol uptake facilitator protein transports arsenite into E.coli. This could help to remove the bottleneck IN As III uptake, potentially improving the efficiency of arsenite remediation1. Thus This bicistronic gene has been optimized to express the glycerol uptake facilitator protein and modified metallothionein IA protein for increased remediation of As III from the environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 183


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Categories
Parameters
None