Part:BBa_K3805209

Bi-stable Switch

The bistable switch is composed of trc promoter(BBa_K3805208))[ https://parts.igem.org/Part:BBa_K3805208], P2 promoter(BBa_I746104))[ https://parts.igem.org/Part:BBa_I746104], lacI- LVA (BBa_C0012))[ https://parts.igem.org/Part:BBa_C0012]and ompT-mcherry(BBa_K3805886))[ https://parts.igem.org/Part:BBa_K3805886]. At the presence of autoinducing peptides(AIPs), P2 promoter will be activated by phosphorylated AgrA (BBa_K38055))[ https://parts.igem.org/Part:BBa_K38055]and initiate the expression of downstrem lacI-LVA and ompT. LacI-LVA inhibits trc promoter.As the concentration of AIP decreases, the downstream transcription of P2 promoter will be down-regulated and the inhibition of lacI-LVA on the trc promoter will thus be lifted.The trc promoter then initiates the its downstream expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1287
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]