Coding

Part:BBa_K4023001

Designed by: Jiachen Lin   Group: iGEM21_Washington   (2021-10-21)
Revision as of 10:22, 21 October 2021 by Registry (Talk | contribs)

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Optimized glpF

glpF codes for a 28kDa transmembrane glycerol uptake facilitator protein found in E.coli strain. glpF protein catalyze transmembrane diffusion of glycerol and some linear polyhydric alcohols in E.coli. glpF protein also allows Arsenite to be transported into E.coli. This is as Arsenite is protonated at physiological pH and forms As(OH)3 as a major species. As(OH)3 is chemically analogous to glycerol.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 183


[edit]
Categories
Parameters
None