Part:BBa_K3805532
OmpT signal peptide
OmpT is one of the four major outer membrane proteases of E. coli. Its protein precursor has a relative molecular mass of 42,000, which is recognized and sheared by the signal skin enzyme during transmembrane, removing 19 amino acid residues from its amino terminus, and processed into a mature outer membrane protease with a relative molecular mass of 40,000, which is secreted into the peripheral lumen of the cell and localized on the outside of the cell membrane. The protease is expressed in high amounts in E. coli and has a secretory effect. The signal skin sequence can be used to construct a secretory prokaryotic expression vector. The OmpT signal skin contains 19 amino acid residues, and two additional amino acid residues were added to the C-terminus of the signal peptide in consideration of the shear recognition amino acid sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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