Coding

Part:BBa_K3806012

Designed by: Laura Sierra Heras   Group: iGEM21_TUDelft   (2021-09-30)
Revision as of 10:14, 21 October 2021 by LauraSH (Talk | contribs)


lacZ reporter gene codon optimized for expression in E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2193
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

BBa_K3806012 contains a codon optimized lacZ gene for optimal expression in E. coli. The lacZ gene encoding β-galactosidase can be used as a colorimetric reporter gene for qualitative and quantitative assays. Several substrates can be used to monitor β-galactosidase activity such O-nitrophenyl β-d-galactopyranoside (ONPG), 5-bromo-4-chloro-3-in-dolyl-β-d-galactoside (X-gal), or chlorophenol red-β-d-galactopyranoside (CPRG). This part is used in BBa_K3806014 and BBa_K3806016 as reporter gene within a aptazyme-regulated genetic circuit that enables a ligand-dependent colorimetric read-out.



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