Composite
davBA-AK

Part:BBa_K3776006

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)
Revision as of 13:32, 17 October 2021 by Meowstarperson (Talk | contribs)


davB-davA-AK

This part allows overproduction of lysine by a feedback insensitive AK and conversion of L-lysine to 5-aminovaleramide (5AVA) by DavB and DavA in Synechococcus elongatus UTEX 2973. Promoters Pcpc560, 6803PsbA2 and terminator TrbcL are from the genome Synechocystis sp. PCC6803.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 800
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1889
    Illegal BglII site found at 4844
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2049
    Illegal NgoMIV site found at 3816
    Illegal AgeI site found at 1456
    Illegal AgeI site found at 1705
    Illegal AgeI site found at 3040
    Illegal AgeI site found at 5043
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None