Composite
T7RNAP-VVD

Part:BBa_K4055001

Designed by: Tianzuo Zeng   Group: iGEM21_Yucai_SZ   (2021-10-21)
Revision as of 09:20, 21 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


J23106-RBS-T7 RNA polymerase N 1~179-Vvd-RBS-Vvd-T7 RNA polymerase C 180~883

This is a similar design to Part:BBa_K3628024 but split the T7RNAP between aa179 and aa180. The split T7RNAP has little activity in a dark environment. When the two segments were exposed to blue light, they will bind together due to the photodimerization of VVD domain and exhibit full RNA polymerase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 1026
    Illegal AgeI site found at 1343
    Illegal AgeI site found at 1571
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/rnap
//rnap/bacteriophage/t7
Parameters
None