Composite

Part:BBa_K3924047

Designed by: Wenda Zhang   Group: iGEM21_Tsinghua   (2021-10-21)
Revision as of 06:39, 21 October 2021 by Registry (Talk | contribs)

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mchE-mchF polycistrons with Ptac lacO promoter

mchE-mchF polycistrons with Ptac lacO promoter

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1713
    Illegal PstI site found at 610
    Illegal PstI site found at 823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1713
    Illegal PstI site found at 610
    Illegal PstI site found at 823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1713
    Illegal BamHI site found at 237
    Illegal BamHI site found at 2380
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1713
    Illegal PstI site found at 610
    Illegal PstI site found at 823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1713
    Illegal PstI site found at 610
    Illegal PstI site found at 823
    Illegal AgeI site found at 2761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1347
    Illegal SapI site found at 2456


[edit]
Categories
Parameters
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