Composite

Part:BBa_K3924048

Designed by: Wenda Zhang   Group: iGEM21_Tsinghua   (2021-10-21)
Revision as of 06:40, 21 October 2021 by Registry (Talk | contribs)

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mchI-mchB-mcmK-mcmL polycistrons with Ptac lacO promoter

mchI-mchB-mcmK-mcmL polycistrons with Ptac lacO promoter

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1663
    Illegal EcoRI site found at 5058
    Illegal SpeI site found at 2422
    Illegal PstI site found at 2368
    Illegal PstI site found at 3293
    Illegal PstI site found at 5269
    Illegal PstI site found at 5278
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1663
    Illegal EcoRI site found at 5058
    Illegal SpeI site found at 2422
    Illegal PstI site found at 2368
    Illegal PstI site found at 3293
    Illegal PstI site found at 5269
    Illegal PstI site found at 5278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1663
    Illegal EcoRI site found at 5058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1663
    Illegal EcoRI site found at 5058
    Illegal SpeI site found at 2422
    Illegal PstI site found at 2368
    Illegal PstI site found at 3293
    Illegal PstI site found at 5269
    Illegal PstI site found at 5278
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1663
    Illegal EcoRI site found at 5058
    Illegal SpeI site found at 2422
    Illegal PstI site found at 2368
    Illegal PstI site found at 3293
    Illegal PstI site found at 5269
    Illegal PstI site found at 5278
    Illegal NgoMIV site found at 3779
    Illegal AgeI site found at 1838
    Illegal AgeI site found at 4501
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None