RNA
7CG
Part:BBa_K3714004
Designed by: Yuxin Lu Group: iGEM21_SJTU-BioX-Shanghai (2021-09-30)
Revision as of 08:22, 21 October 2021 by Ricardo Su (Talk | contribs)
Improved theophylline biosensor
An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1).
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Categories
Parameters
None |