Composite

Part:BBa_K3939998

Designed by: Lingwei Ding   Group: iGEM21_Tianjin   (2021-09-30)
Revision as of 11:35, 30 September 2021 by Registry (Talk | contribs)

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Cas9 with flipped promoter

This part is constructed by two LoxP sequences and a reversed pGal promoter. the pGal promoter is not a tightly regulated promoter. thus,we used estrogen and galactose to induce Cas9 expression, allowing this element to be regulated more precisely.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 749
    Illegal BglII site found at 1686
    Illegal BamHI site found at 2504
    Illegal XhoI site found at 4042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3238
    Illegal AgeI site found at 402
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None