Regulatory

Part:BBa_K3712013

Designed by: Yifan Li   Group: iGEM21_HUST2-China   (2021-10-20)
Revision as of 07:44, 21 October 2021 by YifanLi (Talk | contribs)


pR/pL promotor

USAGE AND RESULTS

PR/PL, a tandem PR and PL promoter from lambda phage, to ensure high-level expression of genes.Our target gene was cloned downstream of the pL and/or pR promoters. When cultured at 37℃, the expression of target gene is inhibited by Tcl and the target protein is hardly expressed. Under the induction of temperature at 45℃ or infrared light which increases temperature above 37℃, Tcl degrades and the inhibition of the promoter releases. So, the expression of target protein is initiated

RESULTS

Figure 1. The agarose gel electrophoresis map of Wild-type BLP-7-27 ELP, Optimized BLP-7-27 ELP, Wild-type TLR2-27ELP, and Optimized TLR2-27 ELP respectively constructed in pR/pL expression system.

Figure 2. About 200-1000 ng of plasmid was digested at 37℃ for 30-60 minutes and analyzed on 1% Agarose Gel. Lane1: circular plasmid (brighter) and supercoiled structure; Lane2: plasmid (brighter) digested by EcoRI and BamHI and target gene fragment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None