Coding

Part:BBa_K3712006

Designed by: Yifan Li   Group: iGEM21_HUST2-China   (2021-10-19)
Revision as of 02:27, 20 October 2021 by Registry (Talk | contribs)

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Optimized TLR2 antagonists

In order to cut off the immune response due to Propionibacterium acnes, we took a small protein sequence of the binding site of TLR2 and P. acnes surface acylated lipoprotein for site-directed mutation to achieve the irreversible binding effect of TLR2 antagonist to acylated lipoprotein, thereby preventing the combination between acylated lipoprotein and TLR2. On the one hand, we increased the hydrophobicity of the hydrophobic pocket bound by the acylated lipoprotein, and on the other hand, we contracted the slack β-loop sheet. In this way, we not only improve the hydrophobic interaction, but also prevent the bound antagonist from going off-target with the acylated lipoprotein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 172


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