Coding
Part:BBa_K3712006
Designed by: Yifan Li Group: iGEM21_HUST2-China (2021-10-19)
Optimized TLR2 antagonists
In order to cut off the immune response due to Propionibacterium acnes, we took a small protein sequence of the binding site of TLR2 and P. acnes surface acylated lipoprotein for site-directed mutation to achieve the irreversible binding effect of TLR2 antagonist to acylated lipoprotein, thereby preventing the combination between acylated lipoprotein and TLR2. On the one hand, we increased the hydrophobicity of the hydrophobic pocket bound by the acylated lipoprotein, and on the other hand, we contracted the slack β-loop sheet. In this way, we not only improve the hydrophobic interaction, but also prevent the bound antagonist from going off-target with the acylated lipoprotein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 172
[edit]
Categories
Parameters
None |