Protein_Domain

Part:BBa_K1781002

Designed by: Marvin Prein   Group: iGEM15_TU_Dresden   (2015-09-09)
Revision as of 05:33, 20 October 2021 by RWSZ (Talk | contribs) (→‎Improvement and New Application for ZHER2)


ZHER2 - affibody with affinity to cytoplasmic domain of HER2

ZHER2 was one of the first affibodies created and it binds to the Human Epidermal Growth Factor 2 (HER2). They originate from mutations in the Immunoglobin G Binding Domain of Protein A from Staphylococcus aureus. Affibodies are engineered to bind to larger proteins or peptides where they imitate monoclonal antibodies. They are used in a multitude of therapies in biotechnology to bioimaging. ZHER2 can particular be used as a protein-protein interaction tool, as well as a way to verify antibody interactions as it binds to the cytoplasmic domain of HER2 at a different epitope than the antibodies.

Improvement and New Application for ZHER2

Group: iGEM2021_Greatbay_SCIE
Author: iGEM2021_Greatbay_SCIE link: Greatbay_SCIE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None