Coding

Part:BBa_K3796002

Designed by: Li Mingyang   Group: iGEM21_CAU_China   (2021-09-04)
Revision as of 16:36, 20 October 2021 by Wubeizhongxinghua (Talk | contribs)


capA from Bacillus subtilis str.168

A key gene for the synthesis of γ-PGA, also named as pgsA, responsible for the transport of γ-PGA to the extracellular compartment.

Usage

capA, capB and capC genes must be used together. When they are expressed together, they may be able to synthesize polyglutamic acid. Three genes can be placed downstream of the same promoter and upstream of the same terminator, or placed separately.

Biology

capABC gene is a gene cluster widely contained in Bacillus sp., which is also named pgsBCA gene cluster. capABC gene cluster can be used to synthesize γ-Polyglutamic acid(γ-PGA) with L-type or D-type glutamate as raw material. Gene capA has catalytic activity and capB has transport activity. The proteins encoded by these three genes work together and can synthesize γ- PGA and transport it to vitro in the form of capsule or free.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None